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BIO170 Lab homework
PART I: LAB ACTIVITY
Directions: This homework assignment will be written in the form of a lab report. The lab report consists of the following sections: introduction, materials/methods, data, results, conclusion, and references. The lab report must be at least two pages in length, double-spaced, Times New Roman, and 12-point font. Below are the various sections. Please structure your lab report so that it includes these sections.
· The introduction mainly consists of three components, background information, objective/purpose of the lab, and hypothesis. Each component should have a paragraph dedicated to it (total of three paragraphs).
o Background information: Research needs to be conducted on what the topic is about.
o Objective/purpose of the lab: You need to clearly define why you are conducting the lab.
o Hypothesis statement: You need to create a hypothesis statement and reasoning for it prior to the start of the lab.
· The materials section lists everything that is needed to complete the experiment. Please include all material that you used.
· The methods section describes all the steps that were taken during the experiment. This section should be written in your own words and not take directly from the lab.
· The data section relays all the data of the lab. They can be presented in charts, tables, etc. The data must be easily read. If there are numerical values, please ensure the correct unit of measurement is displayed as well.
· The results section explains what the data means.
· The conclusion helps sum up and gives meaning to the results. The hypothesis should be restated and confirmed if it was correct or incorrect. Any explanation of why should be given. This explanation of “why” is the driving force of the conclusion. Reference back to your Introduction to help explain data and results. The conclusion should be at least two paragraphs in length.
· A reference section is needed. There should be a total of two references. If you have a reference in this section, it should also have an in-text citation in the lab as well. References can be obtained by using LIRN. Please visit the Academic Resource Center (ARC) for helpful APA format, LIRN, and citation information.
Task/Lab: You will write a lab report for the following task.
Determine if protein is present in various foods.
Protein strengthens your muscles and organs, helps your immune system, and controls many processes inside your cells. Your body also uses specialized protein molecules to make red blood cells, which carry oxygen to all parts of your body. Proteins are large, complex molecules made up of smaller “building blocks” called amino acids. Digestion breaks proteins into individual amino acids that come together to form new proteins that fit the needs of each individual cell. Protein is an essential part of our diet, but because it is complex, it’s difficult to digest, so we can only eat small amounts every day. Protein does not get stored in the body, though, so it is important to eat it regularly. In this experiment, test food for protein using Biuret reagent. Before you begin, form a hypothesis regarding which foods you think contain protein.
Follow these steps:
1. From your Food Chemistry Experiment kit:
· 6 test tubes
· test tube rack
· 6 clean pipets
· wax pencil
· Biuret reagent
2. 6 foods/food solutions. Examples: egg whites (raw), applesauce, whole milk, chocolate milk, cooking oil, etc.
3. Describe all the steps that you plan to take during the experiment and record those steps as the steps are completed.
· Set up six clean test tubes in the rack.
· Choose six food substances and make liquid solutions out of the solid foods by mixing ½ teaspoon of ground food and 1 teaspoon of water. Place one dropper of a solution into each of the test tubes. Using the wax pencil, label each test tube with the name of the solution.
· Record the color of each solution and make a hypothesis about each food’s protein content.
· Put 2 drops of Biuret reagent into each test tube. Carefully swirl the test tubes, watching as the colors change. A bluish-purple color indicates the presence of protein.
4. Record all the results of this experiment.
5. In your conclusion, explain what occurred and if your hypothesis was correct or incorrect. Compare to the results of previous experiments. Answer the following questions about proteins.
· Determine your own protein requirement using the formula: weight in kg x 0.8 g protein = daily protein requirement. (Note: weight in lbs ÷ 2.2 = weight in kg)
· Describe the factors that influence dietary protein needs.
PART II: PROJECT – GLUTEN-FREE MEAL PLAN & SHORT RESPONSE
Gluten-free diets are becoming very popular in our country. Develop a two-day meal plan that is based on a gluten-free diet: one day should be modeled on a typical weekday, and the second day should be modeled on a weekend. Your meal plan should include breakfast, lunch, dinner, and two snacks. Remember that meals should be well-balanced. Please include a restaurant meal in your plan. Your plan must be at least two pages in length, double-spaced, Times New Roman, and 12-point font. Please be sure to cite any references used.
In addition to your two-day meal plan, please write a 100-150 word response to each of the questions below. Please ensure that your responses are written in complete sentences.
1. Do you know anyone who follows a gluten-free diet? If so, what type of medical condition do they have that has restricted them to this diet? If you do not know anyone personally, what types of medical conditions would restrict an individual to this diet?
2. How difficult did you find it to plan the gluten-free meals?
3. Did you have any difficulty planning the gluten-free meal that was eaten out at a restaurant?
4. Were there gluten-free options provided on the menu? If not, what suggestions would you give the restaurant to include gluten-free options on their menu?
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